Perforin and its role in T lymphocyte-mediated cytolysis

Details

Serval ID
serval:BIB_D9DB6EE712A6
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Perforin and its role in T lymphocyte-mediated cytolysis
Journal
Experientia
Author(s)
Lowin  B., Krahenbuhl  O., Muller  C., Dupuis  M., Tschopp  J.
ISSN
0014-4754 (Print)
Publication state
Published
Issued date
10/1992
Volume
48
Number
10
Pages
911-20
Notes
Journal Article
Review --- Old month value: Oct 15
Abstract
The killing mediated by cytotoxic T lymphocytes (CTL) represents an important mechanism in the immune defence against tumors and virus infections. The lytic mechanism has been proposed to consist of a polarized secretion of granule-stored molecules, occurring on effector-target cell contact. By electron microscopy, membrane deposited, pore-like lesions are detected on the target cell membrane during cytolysis by CTL. These structures resembled strikingly pores formed during complement attack. Granules of CTL isolated by nitrogen cavitation and Percoll gradient centrifugation were shown to retain cytotoxic activity. Further purification of proteins stored in these granules led to the discovery of a membranolytic protein named perforin which was capable of polymerizing into pore-like structures. In addition to this cytolytic protein, a set of serine esterases was found as well as lysosomal enzymes and proteoglycans, whose function are not yet clearly defined. The role of perforin in the cytotoxic process is currently being explored by ablating the active gene in mice.
Keywords
Animals Antibody-Dependent Cell Cytotoxicity *Cytotoxicity, Immunologic Exons Humans Killer Cells, Natural/*immunology Membrane Glycoproteins/genetics/*physiology Models, Biological Pore Forming Cytotoxic Proteins T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Create date
24/01/2008 15:18
Last modification date
20/08/2019 15:59
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