Transcriptomic biomarkers of altered erythropoiesis to detect autologous blood transfusion.

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Version: Author's accepted manuscript
Serval ID
serval:BIB_D10E2B16EB63
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Transcriptomic biomarkers of altered erythropoiesis to detect autologous blood transfusion.
Journal
Drug testing and analysis
Author(s)
Salamin O., Mignot J., Kuuranne T., Saugy M., Leuenberger N.
ISSN
1942-7611 (Electronic)
ISSN-L
1942-7603
Publication state
Published
Issued date
03/2018
Peer-reviewed
Oui
Volume
10
Number
3
Pages
604-608
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Autologous blood transfusion is a powerful means of improving performance and remains one of the most challenging methods to detect. Recent investigations have identified 3 candidate reticulocytes genes whose expression was significantly influenced by blood transfusion. Using quantitative reverse transcription polymerase chain reaction as an alternative quantitative method, the present study supports that delta-aminolevulinate synthase 2 (ALAS2), carbonic anhydrase (CA1), and solute carrier family 4 member 1 (SLC4A1) genes are down-regulated post-transfusion. The expression of these genes exhibited stronger correlation with immature reticulocyte fraction than with reticulocytes percentage. Moreover, the repression of reticulocytes' gene expression was more pronounced than the diminution of immature reticulocyte fraction and reticulocyte percentage following blood transfusion. It suggests that the 3 candidate genes are reliable predictors of bone marrow's response to blood transfusion and that they represent potential biomarkers for the detection of this method prohibited in sports.
Keywords
5-Aminolevulinate Synthetase/genetics, Adult, Anion Exchange Protein 1, Erythrocyte/genetics, Blood Transfusion, Autologous/methods, Carbonic Anhydrase I/genetics, Doping in Sports/methods, Down-Regulation, Erythropoiesis, Humans, Male, Reticulocytes/cytology, Reticulocytes/metabolism, Transcriptome, IRF, transcriptomics, transfusion
Pubmed
Web of science
Open Access
Yes
Create date
04/07/2017 14:06
Last modification date
20/08/2019 16:51
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