Inhibition of Ras activity coordinates cell fusion with cell-cell contact during yeast mating.

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State: Public
Version: Final published version
Serval ID
serval:BIB_CFEC44C3FF8F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Inhibition of Ras activity coordinates cell fusion with cell-cell contact during yeast mating.
Journal
The Journal of cell biology
Author(s)
Merlini L., Khalili B., Dudin O., Michon L., Vincenzetti V., Martin S.G.
ISSN
1540-8140 (Electronic)
ISSN-L
0021-9525
Publication state
Published
Issued date
02/04/2018
Peer-reviewed
Oui
Volume
217
Number
4
Pages
1467-1483
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Video-Audio Media
Publication Status: ppublish
Abstract
In the fission yeast Schizosaccharomyces pombe, pheromone signaling engages a signaling pathway composed of a G protein-coupled receptor, Ras, and a mitogen-activated protein kinase (MAPK) cascade that triggers sexual differentiation and gamete fusion. Cell-cell fusion requires local cell wall digestion, which relies on an initially dynamic actin fusion focus that becomes stabilized upon local enrichment of the signaling cascade on the structure. We constructed a live-reporter of active Ras1 (Ras1-guanosine triphosphate [GTP]) that shows Ras activity at polarity sites peaking on the fusion structure before fusion. Remarkably, constitutive Ras1 activation promoted fusion focus stabilization and fusion attempts irrespective of cell pairing, leading to cell lysis. Ras1 activity was restricted by the guanosine triphosphatase-activating protein Gap1, which was itself recruited to sites of Ras1-GTP and was essential to block untimely fusion attempts. We propose that negative feedback control of Ras activity restrains the MAPK signal and couples fusion with cell-cell engagement.
Keywords
Cell Polarity, Enzyme Activation, Feedback, Physiological, GTPase-Activating Proteins/genetics, GTPase-Activating Proteins/metabolism, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Kinetics, Mitogen-Activated Protein Kinases/metabolism, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/growth & development, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Schizosaccharomyces/enzymology, Schizosaccharomyces/genetics, Schizosaccharomyces/growth & development, Schizosaccharomyces pombe Proteins/genetics, Schizosaccharomyces pombe Proteins/metabolism, Signal Transduction, ras Proteins/genetics, ras Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
01/03/2018 20:26
Last modification date
20/08/2019 15:50
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