A combined array-based comparative genomic hybridization and functional library screening approach identifies mir-30d as an oncomir in cancer.
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Download: BIB_CFABD2FA201F.P001.pdf (4042.09 [Ko])
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Version: author
State: Public
Version: author
Serval ID
serval:BIB_CFABD2FA201F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A combined array-based comparative genomic hybridization and functional library screening approach identifies mir-30d as an oncomir in cancer.
Journal
Cancer Research
ISSN
1538-7445 (Electronic)
ISSN-L
0008-5472
Publication state
Published
Issued date
2012
Volume
72
Number
1
Pages
154-164
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
Oncomirs are microRNAs (miRNA) that acts as oncogenes or tumor suppressor genes. Efficient identification of oncomirs remains a challenge. Here we report a novel, clinically guided genetic screening approach for the identification of oncomirs, identifying mir-30d through this strategy. mir-30d regulates tumor cell proliferation, apoptosis, senescence, and migration. The chromosomal locus harboring mir-30d was amplified in more than 30% of multiple types of human solid tumors (n = 1,283). Importantly, higher levels of mir-30d expression were associated significantly with poor clinical outcomes in ovarian cancer patients (n = 330, P = 0.0016). Mechanistic investigations suggested that mir-30d regulates a large number of cancer-associated genes, including the apoptotic caspase CASP3. The guided genetic screening approach validated by this study offers a powerful tool to identify oncomirs that may have utility as biomarkers or targets for drug development.
Keywords
Animals, Apoptosis/genetics, Cell Proliferation, Chromosome Mapping, Comparative Genomic Hybridization, Female, Humans, In Situ Hybridization, Mice, MicroRNAs/genetics, Ovarian Neoplasms/genetics, Ovarian Neoplasms/pathology, Polymorphism, Single Nucleotide, Real-Time Polymerase Chain Reaction
Pubmed
Web of science
Open Access
Yes
Create date
14/10/2014 12:42
Last modification date
20/08/2019 16:50