In vitro transcription and processing of a yeast tRNA gene containing an intervening sequence.

Details

Serval ID
serval:BIB_CF469443D709
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vitro transcription and processing of a yeast tRNA gene containing an intervening sequence.
Journal
Cell
Author(s)
Ogden R.C., Beckman J.S., Abelson J., Kang H.S., Söll D., Schmidt O.
ISSN
0092-8674
Publication state
Published
Issued date
06/1979
Peer-reviewed
Oui
Volume
17
Number
2
Pages
399-406
Language
english
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S. - Publication Status: ppublish
Abstract
A gene for Saccharomyces cerevisiae tRNATrp has been sequenced which contains an intervening sequence of 34 bp (H. S. Kang and J. Abelson, unpublished results). The mutant yeast strain ts-136 accumulates a precursor to tRNATrp which contains mature ends and is colinear with the tRNATrp gene. A nuclear extract from Xenopus oocytes is capable of supporting transcription of the tRNATrp gene contained on plasmid pBR313. The products are precursor tRNAs which contain the intervening RNA sequence. The Xenopus extract accurately splices the precursor transcript to mature-sized tRNATrp.
Keywords
Animals, Cell Nucleus/metabolism, Cell-Free System, Female, Genes, Nucleic Acid Precursors/genetics, Nucleic Acid Precursors/metabolism, Oocytes, RNA, Transfer/genetics, Saccharomyces cerevisiae, Transcription, Genetic, Xenopus
Pubmed
Web of science
Create date
25/01/2008 16:17
Last modification date
20/08/2019 15:49
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