In vitro transcription and processing of a yeast tRNA gene containing an intervening sequence.

Détails

ID Serval
serval:BIB_CF469443D709
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vitro transcription and processing of a yeast tRNA gene containing an intervening sequence.
Périodique
Cell
Auteur(s)
Ogden R.C., Beckman J.S., Abelson J., Kang H.S., Söll D., Schmidt O.
ISSN
0092-8674
Statut éditorial
Publié
Date de publication
06/1979
Peer-reviewed
Oui
Volume
17
Numéro
2
Pages
399-406
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S. - Publication Status: ppublish
Résumé
A gene for Saccharomyces cerevisiae tRNATrp has been sequenced which contains an intervening sequence of 34 bp (H. S. Kang and J. Abelson, unpublished results). The mutant yeast strain ts-136 accumulates a precursor to tRNATrp which contains mature ends and is colinear with the tRNATrp gene. A nuclear extract from Xenopus oocytes is capable of supporting transcription of the tRNATrp gene contained on plasmid pBR313. The products are precursor tRNAs which contain the intervening RNA sequence. The Xenopus extract accurately splices the precursor transcript to mature-sized tRNATrp.
Mots-clé
Animals, Cell Nucleus/metabolism, Cell-Free System, Female, Genes, Nucleic Acid Precursors/genetics, Nucleic Acid Precursors/metabolism, Oocytes, RNA, Transfer/genetics, Saccharomyces cerevisiae, Transcription, Genetic, Xenopus
Pubmed
Web of science
Création de la notice
25/01/2008 17:17
Dernière modification de la notice
20/08/2019 16:49
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