Plasma IGF-1 binding proteins in lean and obese non-diabetic subjects

Details

Serval ID
serval:BIB_CCAF949BB79C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Plasma IGF-1 binding proteins in lean and obese non-diabetic subjects
Journal
International Journal of Obesity and Related Metabolic Disorders
Author(s)
Tappy  L., Jan  C., Temler  E.
ISSN
0307-0565 (Print)
Publication state
Published
Issued date
06/1993
Volume
17
Number
6
Pages
355-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
Obese patients have markedly increased plasma insulin concentrations indicating insulin resistance. In contrast, plasma IGF-1 concentrations are little affected. Since the biological effects of IGF-1, including several insulin-like actions, appear to be modulated by serum carrier proteins, alterations of IGF-1 binding proteins were investigated in the serum of lean healthy individuals, and of obese patients with normal glucose tolerance. Obese patients had increased fasting plasma insulin and decreased plasma h-GH concentrations, compared to lean controls. IGF-1 concentrations were similar in both groups. Serum IGF-1 binding proteins were separated by polyacrylamide gel electrophoresis under non-reducing conditions, electroblotted onto a nitrocellulose sheet, and quantified by ligand blot with 125I IGF-1 and autoradiography. IGF-1 binding proteins of apparent molecular weight 42 kDa, 39 kDa, 35 kDa, 30 kDa and 24 kDa were observed. Obese patients had a level of IGF-1 binding proteins of 35 kDa which was decreased to 40 +/- 25% (s.d.) of control values. This 35 kDa protein corresponds to IGF-BP2, which may be involved in glucose homeostasis. It is concluded that alterations of IGF-1 binding proteins are present in obesity and may have consequences for glucose metabolism.
Keywords
Adult Autoradiography Carrier Proteins/*blood Electrophoresis, Polyacrylamide Gel Female Growth Hormone/blood Humans Insulin/blood Insulin-Like Growth Factor Binding Protein 1 Male Molecular Weight Obesity/*blood
Pubmed
Web of science
Create date
24/01/2008 14:36
Last modification date
03/03/2018 21:28
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