Plasma IGF-1 binding proteins in lean and obese non-diabetic subjects
Détails
ID Serval
serval:BIB_CCAF949BB79C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Plasma IGF-1 binding proteins in lean and obese non-diabetic subjects
Périodique
International Journal of Obesity and Related Metabolic Disorders
ISSN
0307-0565 (Print)
Statut éditorial
Publié
Date de publication
06/1993
Volume
17
Numéro
6
Pages
355-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
Obese patients have markedly increased plasma insulin concentrations indicating insulin resistance. In contrast, plasma IGF-1 concentrations are little affected. Since the biological effects of IGF-1, including several insulin-like actions, appear to be modulated by serum carrier proteins, alterations of IGF-1 binding proteins were investigated in the serum of lean healthy individuals, and of obese patients with normal glucose tolerance. Obese patients had increased fasting plasma insulin and decreased plasma h-GH concentrations, compared to lean controls. IGF-1 concentrations were similar in both groups. Serum IGF-1 binding proteins were separated by polyacrylamide gel electrophoresis under non-reducing conditions, electroblotted onto a nitrocellulose sheet, and quantified by ligand blot with 125I IGF-1 and autoradiography. IGF-1 binding proteins of apparent molecular weight 42 kDa, 39 kDa, 35 kDa, 30 kDa and 24 kDa were observed. Obese patients had a level of IGF-1 binding proteins of 35 kDa which was decreased to 40 +/- 25% (s.d.) of control values. This 35 kDa protein corresponds to IGF-BP2, which may be involved in glucose homeostasis. It is concluded that alterations of IGF-1 binding proteins are present in obesity and may have consequences for glucose metabolism.
Mots-clé
Adult
Autoradiography
Carrier Proteins/*blood
Electrophoresis, Polyacrylamide Gel
Female
Growth Hormone/blood
Humans
Insulin/blood
Insulin-Like Growth Factor Binding Protein 1
Male
Molecular Weight
Obesity/*blood
Pubmed
Web of science
Création de la notice
24/01/2008 14:36
Dernière modification de la notice
20/08/2019 16:47