Membrane mu poly(A) signal and 3' flanking sequences function as a transcription terminator for immunoglobulin-encoding genes.

Details

Serval ID
serval:BIB_CBEB3630DA8A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Membrane mu poly(A) signal and 3' flanking sequences function as a transcription terminator for immunoglobulin-encoding genes.
Journal
Gene
Author(s)
Fasel N.J., Rousseaux M., Déglon N., Govan H.L., Law R., Bron C., Wall R.
ISSN
0378-1119 (Print)
ISSN-L
0378-1119
Publication state
Published
Issued date
1992
Volume
122
Number
2
Pages
297-304
Language
english
Abstract
Developmentally regulated mechanisms involving alternative RNA splicing and/or polyadenylation, as well as transcription termination, are implicated in controlling the levels of secreted mu (mu s), membrane mu (mu m) and delta immunoglobulin (Ig) heavy chain mRNAs during B cell differentiation (mu gene encodes the mu heavy chain). Using expression vectors constructed with genomic DNA segments composed of the mu m polyadenylation signal region, we analyzed poly(A) site utilization and termination of transcription in stably transfected myeloma cells and in murine fibroblast L cells. We found that the gene segment containing the mu m poly(A) signals, along with 536 bp of downstream flanking sequence, acted as a transcription terminator in both myeloma cells and L cell fibroblasts. Neither a 141-bp DNA fragment (which directed efficient polyadenylation at the mu m site), nor the 536-bp flanking nucleotide sequence alone, were sufficient to obtain a similar regulation. This shows that the mu m poly(A) region plays a central role in controlling developmentally regulated transcription termination by blocking downstream delta gene expression. Because this gene segment exhibited the same RNA processing and termination activities in fibroblasts, it appears that these processes are not tissue-specific.
Keywords
Alternative Splicing, Animals, Base Sequence, Blotting, Northern, Gene Expression Regulation, Genes, Immunoglobulin, Genetic Vectors, Humans, Immunoglobulin mu-Chains/genetics, L Cells (Cell Line), Mice, Molecular Sequence Data, Poly A, Terminator Regions, Genetic, Tumor Cells, Cultured
Pubmed
Web of science
Create date
24/01/2008 16:02
Last modification date
20/08/2019 16:46
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