False human cytomegalovirus IgG-positivity at prenatal screening
Details
Serval ID
serval:BIB_CB60C458FCA5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
False human cytomegalovirus IgG-positivity at prenatal screening
Journal
J Clin Virol
ISSN
1386-6532
Publication state
Published
Issued date
07/2018
Volume
104
Pages
34-38
Language
english
Notes
1873-5967
Furione, Milena
Sarasini, Antonella
Arossa, Alessia
Fornara, Chiara
Lilleri, Daniele
Perez, Laurent
Parea, Maurizio
Zavattoni, Maurizio
Spinillo, Arsenio
Marone, Piero
Baldanti, Fausto
Journal Article
Research Support, Non-U.S. Gov't
Netherlands
J Clin Virol. 2018 Jul;104:34-38. doi: 10.1016/j.jcv.2018.04.009. Epub 2018 Apr 21.
Furione, Milena
Sarasini, Antonella
Arossa, Alessia
Fornara, Chiara
Lilleri, Daniele
Perez, Laurent
Parea, Maurizio
Zavattoni, Maurizio
Spinillo, Arsenio
Marone, Piero
Baldanti, Fausto
Journal Article
Research Support, Non-U.S. Gov't
Netherlands
J Clin Virol. 2018 Jul;104:34-38. doi: 10.1016/j.jcv.2018.04.009. Epub 2018 Apr 21.
Abstract
BACKGROUND: An incorrect definition of immune status to human cytomegalovirus (HCMV) can lead to incorrect management of pregnant women. OBJECTIVES: Aims of the study were: i) to describe 10 cases of unconfirmed HCMV IgG-seroconversion in pregnancy; ii) to develop a panel of confirmatory tests to define HCMV serostatus; iii) to investigate the frequency of false IgG-positive results in pregnant women screened with the LIAISON(®)CMVIgGII automated assay. STUDY DESIGN: Blood samples from 10 pregnant women referred for HCMV IgG-seroconversion were examined to confirm/exclude a primary infection. In addition, samples were tested for HCMV IgG by immunoblotting, neutralization assay, and ELISA against gB, gH/gL/pUL128L and gH/gL/gO recombinant glycoproteins. LIAISON(®)CMVIgGII results obtained on 1158 pregnant women were reviewed and samples with low IgG titers were further investigated. RESULTS: A primary infection was excluded in the 10 women referred for HCMV IgG seroconversion. None of them was confirmed to be IgG-seropositive. Of the 1158 women prenatally screened by LIAISON(®)CMVIgGII, 678 (59%) were IgG-positive and, of these, 40 (5.9%) showed low levels of IgG (14-50 U/mL). Thirty-three women with low IgG-positivity were further tested by confirmatory tests and 11 (33.3%) were found to be non reactive to HCMV. CONCLUSIONS: At least 1.6% (11/678) women who tested positive with LIAISON(®)CMVIgGII were found to be seronegative when tested with confirmatory tests. These women should be informed to reduce the risk of a primary HCMV infection. Furthermore, should a congenital infection occur in any of these women, a maternal non-primary infection could be erroneously diagnosed.
Keywords
Antibodies, Viral/*blood, Cytomegalovirus/*immunology, Cytomegalovirus Infections/*diagnosis, *False Positive Reactions, Female, Humans, Immunoglobulin G/*blood, Pregnancy, *Prenatal Diagnosis, Prevalence, Retrospective Studies, *Confirmatory tests, *Fully automatized assays, *Human cytomegalovirus, *Non-primary infection, *Pregnancy, *Prenatal screening
Create date
04/09/2020 20:03
Last modification date
07/09/2020 6:26