Oligoclonal expansion of major histocompatibility complex class I-restricted cytolytic T lymphocytes during a primary immune response in vivo: direct monitoring by flow cytometry and polymerase chain reaction.
Details
Request a copy Under indefinite embargo.
UNIL restricted access
State: Public
Version: Final published version
License: Not specified
UNIL restricted access
State: Public
Version: Final published version
License: Not specified
Serval ID
serval:BIB_CAD587898C42
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Oligoclonal expansion of major histocompatibility complex class I-restricted cytolytic T lymphocytes during a primary immune response in vivo: direct monitoring by flow cytometry and polymerase chain reaction.
Journal
The Journal of experimental medicine
ISSN
0022-1007
ISSN-L
0022-1007
Publication state
Published
Issued date
01/05/1993
Peer-reviewed
Oui
Volume
177
Number
5
Pages
1487-1492
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Previous T cell receptor (TCR) sequence analysis of a panel of 23 H-2Kd restricted cytotoxic T lymphocyte (CTL) clones recognizing the decapeptide HLA-CW3 170-179 revealed a striking conservation of TCR structure, in that all clones examined used V beta 10 and J alpha pHDS58 segments. We show here that the primary response in vivo after intraperitoneal injection of DBA/2 mice with HLA-CW3 expressing transfectants of syngeneic P815 (H-2d) tumor cells is characterized by a dramatic expansion of CD8+ V beta 10+ CTL in the peritoneal cavity and draining (mesenteric) lymph node, as well as in peripheral blood. Additional analysis of TCR on HLA-CW3 immune populations by flow cytometry and polymerase chain reaction further indicates that the vast majority of responding CD8+ cells express restricted V alpha domains, a dominant J alpha segment (pHDS58), and a conserved CDR3 length for both alpha and beta chains. This novel system provides a unique opportunity to directly monitor an oligoclonal primary antigen specific immune response in vivo at the single cell level independently of functional assays.
Keywords
Animals, CD8 Antigens, Cells, Cultured, Clone Cells, Female, Flow Cytometry, HLA-C Antigens/immunology, Histocompatibility Antigens Class I/immunology, Mice, Mice, Inbred DBA, Peptide Fragments/immunology, Polymerase Chain Reaction, Receptors, Antigen, T-Cell, alpha-beta, T-Lymphocytes, Cytotoxic/cytology, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:13
Last modification date
09/08/2024 15:51