In vitro renin inhibition to prevent generation of angiotensins during determination of angiotensin I and II

Details

Serval ID
serval:BIB_C874ABA44F39
Type
Article: article from journal or magazin.
Collection
Publications
Title
In vitro renin inhibition to prevent generation of angiotensins during determination of angiotensin I and II
Journal
Life Sciences
Author(s)
Nussberger  J., Brunner  D. B., Waeber  B., Brunner  H. R.
ISSN
0024-3205 (Print)
Publication state
Published
Issued date
1988
Volume
42
Number
17
Pages
1683-8
Notes
Journal Article
Abstract
To prevent in vitro generation of angiotensins, the renin inhibitor CGP 29287 (CGP) was added to blood sampling tubes. Plasma immunoreactive angiotensin (ir-ANG) I and II were simultaneously measured by radioimmunoassay after rapid and quantitative extraction from a single plasma sample on phenylsilylsilica (Bondelut PH). True plasma ANG-(1-8)octapeptide was determined after additional separation of the different angiotensins by high performance liquid chromatography. Ir-ANG II/CGP showed the known linear relationship with ANG-(1-8)octapeptide (r = 0.87, n = 23), but - in contrast to studies without addition of CGP - the y-axis intercept which presumably represents cross-reacting angiotensins other than ANG II was very small. Ir-ANG II/CGP concentrations fell below 1 fmol/ml after converting enzyme inhibition. The results suggest that CGP 29287 prevents in vitro generation of ANG I and ANG II as well as the ANG-metabolites. Ir-ANG I/CGP measured after Bondelut PH extraction of the plasma was strongly correlated with ir-ANG I obtained after blood ethanol extraction (r = 0.97, n = 23). Thus, it is now possible to measure reliably both ANG I and ANG II within the same plasma extract after a simple extraction procedure.
Keywords
Angiotensin I/*blood Angiotensin II/*blood Chromatography, High Pressure Liquid Enalapril/diagnostic use Humans *Oligopeptides Radioimmunoassay Renin/*antagonists & inhibitors/blood/pharmacology
Pubmed
Web of science
Create date
05/03/2008 17:39
Last modification date
20/08/2019 16:43
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