Biochemical quantification of total brain glycogen concentration in rats under different glycemic states.

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Serval ID
serval:BIB_C81559D67CD6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Biochemical quantification of total brain glycogen concentration in rats under different glycemic states.
Journal
Neurochemistry International
Author(s)
Morgenthaler F.D., Koski D.M., Kraftsik R., Henry P.G., Gruetter R.
ISSN
0197-0186 (Print)
ISSN-L
0197-0186
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
48
Number
6-7
Pages
616-622
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
All (13)C NMR studies of brain glycogen to date relied on observing the incorporation of (13)C label into glycogen, and thus interpretation was potentially affected by changes in (13)C label turnover rates. The goal of this study was to quantify total brain glycogen concentration under conditions of hypoglycemia or normoglycemia using biochemical methods. Rats were sacrificed using a focused microwave fixation device. The results showed that metabolism of brain glycogen was Glc- and insulin-sensitive and that insulin-induced hypoglycemia promoted a gradual glycogenolysis. Moreover, we show that there are very mild effects of isoflurane and alpha-chloralose anesthesia on brain glycogen concentration. Altogether these results show that total brain glycogen serves as a substantial source of glucosyl units during insulin-induced moderate hypoglycemia and therefore may be neuroprotective. Finally we also conclude that previous interpretation of (13)C NMR spectroscopy data accurately reflected the changes in total brain glycogen content.
Keywords
Animals, Blood Glucose/metabolism, Brain/metabolism, Glycogen/metabolism, Hypoglycemia/metabolism, Insulin/metabolism, Magnetic Resonance Spectroscopy, Male, Rats, Rats, Sprague-Dawley
Pubmed
Web of science
Create date
26/02/2008 16:25
Last modification date
20/08/2019 15:43
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