Fading and rebound of P2X2 currents at millimolar ATP concentrations caused by low pH.

Details

Ressource 1Request a copy Sous embargo indéterminé.
State: Public
Version: author
Serval ID
serval:BIB_C1A78ED8BEF1
Type
Article: article from journal or magazin.
Collection
Publications
Title
Fading and rebound of P2X2 currents at millimolar ATP concentrations caused by low pH.
Journal
British Journal of Pharmacology
Author(s)
Stoop R., Quayle J.M.
ISSN
0007-1188 (Print)
ISSN-L
0007-1188
Publication state
Published
Issued date
1998
Peer-reviewed
Oui
Volume
125
Number
2
Pages
235-237
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
When ATP is applied at high concentrations (above 1 mM) to PC12 cells, it produces a rapidly desensitizing peak current followed by a rebound of the current after termination of the ATP application. We expressed P2X2 receptors, which are thought to mediate the ATP currents of PC12 cells, in HEK293 cells and studied the effects of acidification on this 'fading and rebound' phenomenon. We found that the desensitization disappeared after adjusting the low pH (<5.0) of the millimolar ATP concentrations to a more physiological value (pH 7.3). Furthermore, the fading and rebound could also be induced at much lower ATP concentrations by decreasing the pH of the ATP containing application solutions. Thus, it appears this phenomenon is not caused directly by high concentrations of ATP, but is due to a concomitant acidification that occurs when high concentrations of ATP are dissolved in only moderately buffered application solutions.
Keywords
Adenosine Triphosphate/metabolism, Animals, Hydrogen-Ion Concentration, Ligands, PC12 Cells, Rats, Receptors, Purinergic P2/drug effects, Receptors, Purinergic P2/metabolism, Receptors, Purinergic P2X2, Tumor Cells, Cultured
Pubmed
Open Access
Yes
Create date
29/10/2013 11:42
Last modification date
20/08/2019 16:36
Usage data