One Year Genome Evolution of Lausannevirus in Allopatric versus Sympatric Conditions.

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Version: Final published version
Serval ID
serval:BIB_BF5DFD01F732
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
One Year Genome Evolution of Lausannevirus in Allopatric versus Sympatric Conditions.
Journal
Genome biology and evolution
Author(s)
Mueller L., Bertelli C., Pillonel T., Salamin N., Greub G.
ISSN
1759-6653 (Electronic)
ISSN-L
1759-6653
Publication state
Published
Issued date
01/06/2017
Peer-reviewed
Oui
Volume
9
Number
6
Pages
1432-1449
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Amoeba-resisting microorganisms raised a great interest during the last decade. Among them, some large DNA viruses present huge genomes up to 2.5 Mb long, exceeding the size of small bacterial genomes. The rate of genome evolution in terms of mutation, deletion, and gene acquisition in these genomes is yet unknown. Given the suspected high plasticity of viral genomes, the microevolution of the 346 kb genome of Lausannevirus, a member of Megavirales, was studied. Hence, Lausannevirus was co-cultured within the amoeba Acanthamoeba castellanii over one year. Despite a low number of mutations, the virus showed a genome reduction of 3.7% after 12 months. Lausannevirus genome evolution in sympatric conditions was investigated by its co-culture with Estrella lausannensis, an obligate intracellular bacterium, in the amoeba A. castellanii during one year. Cultures were split every 3 months. Genome sequencing revealed that in these conditions both, Lausannevirus and E. lausannensis, show stable genome, presenting no major rearrangement. In fact, after one year they acquired from 2 to 7 and from 4 to 10 mutations per culture for Lausannevirus and E. lausannensis, respectively. Interestingly, different mutations in the endonuclease encoding genes of Lausannevirus were observed in different subcultures, highlighting the importance of this gene product in the replication of Lausannevirus. Conversely, mutations in E. lausannensis were mainly located in a gene encoding for a phosphoenolpyruvate-protein phosphotransferase (PtsI), implicated in sugar metabolism. Moreover, in our conditions and with our analyses we detected no horizontal gene transfer during one year of co-culture.

Keywords
Chlamydiales, Lausannevirus, Marseilleviridae, Megavirales, experimental evolution, single nucleotide polymorphisms (SNPs)
Pubmed
Web of science
Open Access
Yes
Create date
30/05/2017 16:33
Last modification date
20/08/2019 15:33
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