Y chromosome microsatellite isolation from BAC clones in the greater white-toothed shrew (Crocidura russula)

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serval:BIB_BD9F535D925F
Type
Article: article from journal or magazin.
Collection
Publications
Title
Y chromosome microsatellite isolation from BAC clones in the greater white-toothed shrew (Crocidura russula)
Journal
Molecular Ecology Notes
Author(s)
Lawson Handley L.J., Perrin N.
ISSN
1471-8278
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
60
Number
1
Pages
276-279
Language
english
Abstract
We constructed a microsatellite library from four Crocidura russula Y chromosome-specific bacterial artificial chromosome (BAC) clones. Only one of eight microsatellites was male-specific, despite genome walking to obtain more flanking sequence and testing of 93 primer combinations. Potential reasons for this low success are discussed. The male-specific locus, CRY3, was genotyped in 90 males, including C. russula from across the species range and two related species. The large difference in CRY3 allele size between eastern and western lineages supports earlier reports of high divergence between them. Despite polymorphism of CRY3 in Morocco, only one allele was found throughout the whole of Europe, consistent with previous studies that suggest recent colonization of Europe from a small number of Moroccan founders.
Keywords
bac library, chromosome, microsatellite library, retro-elements, shrews
Web of science
Open Access
Yes
Create date
19/11/2007 11:46
Last modification date
20/08/2019 16:31
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