Evolutionary patchwork of an insecticidal toxin shared between plant-associated pseudomonads and the insect pathogens Photorhabdus and Xenorhabdus.

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Version: Final published version
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Serval ID
serval:BIB_BD1F089A09CB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Evolutionary patchwork of an insecticidal toxin shared between plant-associated pseudomonads and the insect pathogens Photorhabdus and Xenorhabdus.
Journal
Bmc Genomics
Author(s)
Ruffner B., Péchy-Tarr M., Höfte M., Bloemberg G., Grunder J., Keel C. (co-last), Maurhofer M.
ISSN
1471-2164 (Electronic)
ISSN-L
1471-2164
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
16
Number
1
Pages
609
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
BACKGROUND: Root-colonizing fluorescent pseudomonads are known for their excellent abilities to protect plants against soil-borne fungal pathogens. Some of these bacteria produce an insecticidal toxin (Fit) suggesting that they may exploit insect hosts as a secondary niche. However, the ecological relevance of insect toxicity and the mechanisms driving the evolution of toxin production remain puzzling.
RESULTS: Screening a large collection of plant-associated pseudomonads for insecticidal activity and presence of the Fit toxin revealed that Fit is highly indicative of insecticidal activity and predicts that Pseudomonas protegens and P. chlororaphis are exclusive Fit producers. A comparative evolutionary analysis of Fit toxin-producing Pseudomonas including the insect-pathogenic bacteria Photorhabdus and Xenorhadus, which produce the Fit related Mcf toxin, showed that fit genes are part of a dynamic genomic region with substantial presence/absence polymorphism and local variation in GC base composition. The patchy distribution and phylogenetic incongruence of fit genes indicate that the Fit cluster evolved via horizontal transfer, followed by functional integration of vertically transmitted genes, generating a unique Pseudomonas-specific insect toxin cluster.
CONCLUSIONS: Our findings suggest that multiple independent evolutionary events led to formation of at least three versions of the Mcf/Fit toxin highlighting the dynamic nature of insect toxin evolution.
Keywords
Animals, Bacterial Toxins/genetics, Evolution, Molecular, Gene Transfer, Horizontal, Insecticides/pharmacology, Insects/microbiology, Multigene Family, Photorhabdus/genetics, Photorhabdus/metabolism, Phylogeny, Plants/microbiology, Pseudomonas fluorescens/genetics, Pseudomonas fluorescens/metabolism, Xenorhabdus/genetics, Xenorhabdus/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
07/07/2015 11:58
Last modification date
13/02/2020 6:19
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