Enhanced Respiratory Chain Supercomplex Formation in Response to Exercise in Human Skeletal Muscle.

Details

Ressource 1Download: Serval_PostPrint_Enhanced Respiratory Supercomplexes.pdf (23732.81 [Ko])
State: Public
Version: Author's accepted manuscript
License: Not specified
Serval ID
serval:BIB_BA4CF7B58271
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Enhanced Respiratory Chain Supercomplex Formation in Response to Exercise in Human Skeletal Muscle.
Journal
Cell metabolism
Author(s)
Greggio C., Jha P., Kulkarni S.S., Lagarrigue S., Broskey N.T., Boutant M., Wang X., Conde Alonso S., Ofori E., Auwerx J., Cantó C., Amati F.
ISSN
1932-7420 (Electronic)
ISSN-L
1550-4131
Publication state
Published
Issued date
07/02/2017
Peer-reviewed
Oui
Volume
25
Number
2
Pages
301-311
Language
english
Notes
Publication types: Clinical Trial ; Journal Article
Publication Status: ppublish
Abstract
Mitochondrial dysfunction is a hallmark of multiple metabolic complications. Physical activity is known to increase mitochondrial content in skeletal muscle, counteracting age-related decline in muscle function and protecting against metabolic and cardiovascular complications. Here, we investigated the effect of 4 months of exercise training on skeletal muscle mitochondria electron transport chain complexes and supercomplexes in 26 healthy, sedentary older adults. Exercise differentially modulated respiratory complexes. Complex I was the most upregulated complex and not stoichiometrically associated to the other complexes. In contrast to the other complexes, complex I was almost exclusively found assembled in supercomplexes in muscle mitochondria. Overall, supercomplex content was increased after exercise. In particular, complexes I, III, and IV were redistributed to supercomplexes in the form of I+III2+IV. Taken together, our results provide the first evidence that exercise affects the stoichiometry of supercomplex formation in humans and thus reveal a novel adaptive mechanism for increased energy demand.
Keywords
Adiposity, Aged, Aging/metabolism, Cell Respiration, Electron Transport Chain Complex Proteins/metabolism, Exercise/physiology, Female, Humans, Male, Middle Aged, Muscle, Skeletal/physiology, Oxygen/metabolism, aging, electron transport chain, endurance training, exercise intervention, human, mitochondria, pre-/post-design, respiratory complex, skeletal muscle, supercomplex
Pubmed
Web of science
Open Access
Yes
Funding(s)
Swiss National Science Foundation / Careers / PZ00P3-149398
Swiss National Science Foundation / Projects / 320030_170062
Swiss National Science Foundation / Careers / PZ00P3-126339
Create date
06/12/2016 18:07
Last modification date
20/01/2020 6:20
Usage data