Nerve growth factor (NGF) stimulation of cholinergic telencephalic neurons in aggregating cell cultures.

Details

Serval ID
serval:BIB_B9239B800012
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nerve growth factor (NGF) stimulation of cholinergic telencephalic neurons in aggregating cell cultures.
Journal
Developmental Brain Research
Author(s)
Honegger P., Lenoir D.
ISSN
0165-3806
ISSN-L
0006-8993
Publication state
Published
Issued date
1982
Volume
3
Number
2
Pages
229-238
Language
english
Abstract
The addition of nerve growth factor (2.5S NGF) to serum-free aggregating cell cultures of fetal rat telencephalon greatly stimulated the developmental increase in choline acetyltransferase activity. Two other neuronal enzymes, acetylcholinesterase and glutamic acid decarboxylase, showed only slightly increased activities after NGF treatment whereas the total protein content of the cultures and the activity of 2',3'- cyclic nucleotide phosphodiesterase remained unchanged. The stimulation of choline acetyltransferase was dependent on the NGF media concentrations, showing a 50% maximum effect (120% increase) at approximately 3 ng/ml (10-10 M 2.5S NGF). NGF treatments during different culture periods showed that the cholinergic neurons remained responsive for at least 19 days. The continued treatment was the most effective; however, an initial treatment for only 5 days still caused a significant stimulation of choline acetyltransferase on day 19. The observed stimulation appeared to be specific to NGF. Univalent antibody fragments (Fab) against 2.5S NGF completely abolished the NGF-dependent increase in choline acetyltransferase activity, whereas Fab fragments of control IgG were ineffective. Furthermore, angiotensin II, added in high amounts to the cultures, showed no stimulatory effect. The present results suggest that certain populations of rat brain neurons are responsive to nerve growth factor.
Keywords
Acetylcholinesterase/metabolism, Animals, Cell Differentiation/drug effects, Cells, Cultured, Choline O-Acetyltransferase/metabolism, Cholinergic Fibers/drug effects, Glutamate Decarboxylase/metabolism, Nerve Growth Factors/pharmacology, Rats, Rats, Inbred Strains, Telencephalon/cytology, Telencephalon/embryology
Pubmed
Web of science
Create date
24/01/2008 13:11
Last modification date
20/08/2019 15:27
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