Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity.
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Version: Final published version
State: Public
Version: Final published version
Serval ID
serval:BIB_B463362881C6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity.
Journal
Breeding Science
ISSN
1344-7610 (Print)
ISSN-L
1344-7610
Publication state
Published
Issued date
2015
Volume
65
Number
4
Pages
327-332
Language
english
Abstract
The cuticle covers the aerial parts of land plants, where it serves many important functions, including water retention. Here, a recessive cuticle mutant, eceriferum-ym (cer-ym), of Hordeum vulgare L. (barley) showed abnormally glossy spikes, sheaths, and leaves. The cer-ym mutant plant detached from its root system was hypersensitive to desiccation treatment compared with wild type plants, and detached leaves of mutant lost 41.8% of their initial weight after 1 h of dehydration under laboratory conditions, while that of the wild type plants lost only 7.1%. Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining. The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H. The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.
Keywords
eceriferum-mutant, cuticle, cutin, desiccation resistance, genetic mapping, wax
Pubmed
Web of science
Open Access
Yes
Create date
13/11/2015 8:06
Last modification date
20/08/2019 15:22