Seed-specific silencing of OsMRP5 reduces seed phytic acid and weight in rice.

Details

Serval ID
serval:BIB_B34B61DD77E8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Seed-specific silencing of OsMRP5 reduces seed phytic acid and weight in rice.
Journal
Transgenic Research
Author(s)
Li W.X., Zhao H.J., Pang W.Q., Cui H.R., Poirier Y., Shu Q.Y.
ISSN
1573-9368 (Electronic)
ISSN-L
0962-8819
Publication state
Published
Issued date
2014
Volume
23
Number
4
Pages
585-599
Language
english
Abstract
Phytic acid (PA) is poorly digested by humans and monogastric animals and negatively affects human/animal nutrition and the environment. Rice mutants with reduced PA content have been developed but are often associated with reduced seed weight and viability, lacking breeding value. In the present study, a new approach was explored to reduce seed PA while attaining competitive yield. The OsMRP5 gene, of which mutations are known to reduce seed PA as well as seed yield and viability, was down-regulated specifically in rice seeds by using an artificial microRNA driven by the rice seed specific promoter Ole18. Seed PA contents were reduced by 35.8-71.9% in brown rice grains of transgenic plants compared to their respective null plants (non-transgenic plants derived from the same event). No consistent significant differences of plant height or number of tillers per plant were observed, but significantly lower seed weights (up to 17.8% reduction) were detected in all transgenic lines compared to null plants, accompanied by reductions of seed germination and seedling emergence. It was observed that the silencing of the OsMRP5 gene increased the inorganic P (Pi) levels (up to 7.5 times) in amounts more than the reduction of PA-P in brown rice. This indicates a reduction in P content in other cellular compounds, such as lipids and nucleic acids, which may affect overall seed development. Put together, the present study demonstrated that seed specific silencing of OsMRP5 could significantly reduce the PA content and increase Pi levels in seeds; however, it also significantly lowers seed weight in rice. Discussions were made regarding future directions towards producing agronomically competitive and nutritionally valuable low PA rice.
Keywords
Artificial microRNA, Seed specific promoter Ole18, Low phytic acid (LPA), Seed viability, OsMRP5, Oryza sativa L.
Pubmed
Web of science
Create date
21/08/2014 9:57
Last modification date
20/08/2019 16:21
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