Generation of cells committed towards the photoreceptor fate for retinal transplantation

Details

Serval ID
serval:BIB_B2F8568E9554
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Generation of cells committed towards the photoreceptor fate for retinal transplantation
Journal
Neuroreport
Author(s)
Canola  K., Arsenijevic  Y.
ISSN
0959-4965 (Print)
Publication state
Published
Issued date
06/2007
Volume
18
Number
9
Pages
851-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun 11
Abstract
Cell transplantation is an active field of research to replace lost cells in retinal dystrophies to potentially restore visual function. We hypothesized that in-vitro differentiated retinal stem cells would integrate the appropriate retinal layer and differentiate into photoreceptors when transplanted during development. Here we show that retinal stem cells driven to the photoreceptor fate start to incorporate the retina and express photoreceptor markers but do not survive. Nevertheless surviving grafted cells express the glial marker glial fibrillary acidic protein and incorporate the ganglion cell layer as well as the inner plexiform layer. These results suggest that the maturation state of the photoreceptors is primordial to obtain robust incorporation and that a fine tuning of retinal stem cells differentiation should provide adequate cells for transplantation.
Keywords
3' Untranslated Regions Animals Cell Differentiation/physiology Glial Fibrillary Acidic Protein/physiology Immunohistochemistry Mice Mice, Inbred C57BL Mice, Knockout Neuroglia/physiology Photoreceptors, Vertebrate/*cytology Retina/*physiology/*transplantation Retinal Ganglion Cells/physiology/transplantation Rhodopsin/genetics/physiology Stem Cells/*physiology
Pubmed
Web of science
Create date
28/01/2008 12:31
Last modification date
20/08/2019 15:21
Usage data