Comparative Genomics Analysis of Streptococcus tigurinus Strains Identifies Genetic Elements Specifically and Uniquely Present in Highly Virulent Strains.

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State: Public
Version: Final published version
Serval ID
serval:BIB_B14812A32BFE
Type
Article: article from journal or magazin.
Collection
Publications
Title
Comparative Genomics Analysis of Streptococcus tigurinus Strains Identifies Genetic Elements Specifically and Uniquely Present in Highly Virulent Strains.
Journal
PloS one
Author(s)
Diene S.M., François P., Zbinden A., Entenza J.M., Resch G.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
11
Number
8
Pages
e0160554
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: epublish
Abstract
Streptococcus tigurinus is responsible for severe invasive infections such as infective endocarditis, spondylodiscitis and meningitis. As described, S. tigurinus isolates AZ_3aT and AZ_14 were highly virulent (HV phenotype) in an experimental model of infective endocarditis and showed enhanced adherence and invasion of human endothelial cells when compared to low virulent S. tigurinus isolate AZ_8 (LV phenotype). Here, we sought whether genetic determinants could explain the higher virulence of AZ_3aT and AZ_14 isolates. Several genetic determinants specific to the HV strains were identified through extensive comparative genomics amongst which some were thought to be highly relevant for the observed HV phenotype. These included i) an iron uptake and metabolism operon, ii) an ascorbate assimilation operon, iii) a newly acquired PI-2-like pilus islets described for the first time in S. tigurinus, iv) a hyaluronate metabolism operon, v) an Entner-Doudoroff pathway of carbohydrates metabolism, and vi) an alternate pathways for indole biosynthesis. We believe that the identified genomic features could largely explain the phenotype of high infectivity of the two HV S. tigurinus strains. Indeed, these features include determinants that could be involved at different stages of the disease such as survival of S. tigurinus in blood (iron uptake and ascorbate metabolism operons), initial attachment of bacterial pathogen to the damaged cardiac tissue and/or vegetation that formed on site (PI-2-like pilus islets), tissue invasion (hyaluronate operon and Entner-Doudoroff pathway) and regulation of pathogenicity (indole biosynthesis pathway).

Keywords
Ascorbic Acid/metabolism, Bacterial Adhesion/genetics, Biological Transport/genetics, Genomics, Hyaluronic Acid/metabolism, Indoles/metabolism, Iron/metabolism, Molecular Sequence Annotation, Phenotype, Proteomics, Species Specificity, Streptococcus/genetics, Streptococcus/metabolism, Streptococcus/pathogenicity, Tryptophan/metabolism, Virulence/genetics
Pubmed
Web of science
Open Access
Yes
Create date
30/09/2016 8:03
Last modification date
20/08/2019 16:20
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