Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.
Details
State: Public
Version: Final published version
Serval ID
serval:BIB_AF335B6D869D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.
Journal
Plos One
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
02/2014
Peer-reviewed
Oui
Volume
9
Number
2
Pages
e88146
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: epublish
Abstract
Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We assembled TALENs and designed a linear donor insert targeting a pA476T mutation in the rat Glucocorticoid Receptor (Nr3c1) namely GR(dim), that prevents receptor homodimerization in the mouse. TALEN mRNA and linear double-stranded donor were microinjected into rat one-cell embryos. Overall, we observed targeted genomic modifications in 17% of the offspring, indicating high TALEN cutting efficiency in rat zygotes.
Pubmed
Open Access
Yes
Create date
05/09/2014 16:57
Last modification date
17/09/2020 9:22