Feedback control of DnaA-mediated replication initiation by replisome-associated HdaA protein in Caulobacter.
Details
Serval ID
serval:BIB_AF078859333C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Feedback control of DnaA-mediated replication initiation by replisome-associated HdaA protein in Caulobacter.
Journal
Journal of bacteriology
ISSN
1098-5530[electronic]
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
191
Number
18
Pages
5706-5716
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
Chromosome replication in Caulobacter crescentus is tightly regulated to ensure that initiation occurs at the right time and only once during the cell cycle. The timing of replication initiation is controlled by both CtrA and DnaA. CtrA binds to and silences the origin. Upon the clearance of CtrA from the cell, the DnaA protein accumulates and allows loading of the replisome at the origin. Here, we identify an additional layer of replication initiation control that is mediated by the HdaA protein. In Escherichia coli, the Hda protein inactivates DnaA after replication initiation. We show that the Caulobacter HdaA homologue is necessary to restrict the initiation of DNA replication to only once per cell cycle and that it dynamically colocalizes with the replisome throughout the cell cycle. Moreover, the transcription of hdaA is directly activated by DnaA, providing a robust feedback regulatory mechanism that adjusts the levels of HdaA to inactivate DnaA.
Pubmed
Web of science
Open Access
Yes
Create date
31/08/2009 14:52
Last modification date
20/08/2019 16:18