Cellular localization, expression and regulation of neuropeptide Y in kidneys of hypertensive rats.

Details

Serval ID
serval:BIB_ADF7EF5E7675
Type
Article: article from journal or magazin.
Collection
Publications
Title
Cellular localization, expression and regulation of neuropeptide Y in kidneys of hypertensive rats.
Journal
Regulatory peptides
Author(s)
Haefliger J.A., Waeber B., Grouzmann E., Braissant O., Nussberger J., Nicod P., Waeber G.
ISSN
0167-0115
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
82
Number
1-3
Pages
35-43
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
Neuropeptide Y (NPY) is a key modulator of the autonomic nervous system playing pivotal roles in cardiovascular and neuronal functions. In this study, we assessed the cellular localization and gene expression of NPY in rat kidneys. We also examined the relationship between NPY gene expression and renin in two rat models of hypertension (two-kidney, one-clip renal hypertension (2K1C), and deoxycorticosterone-salt-induced hypertension (DOCA-salt)) characterized by a similar blood pressure elevation. In situ hybridization and immunohistochemistry, using anti-NPY or anti-C-flanking peptide of NPY (CPON) antibodies, showed that NPY transcript and protein were colocalized in the tubules of rat kidneys. During experimental hypertension, NPY mRNA was decreased in both kidneys of the 2K1C animals, but not in the kidney of DOCA-salt rats. In 2K1C rats, renal NPY content was also decreased. The difference in NPY gene expression between 2K1C rats (a high renin model of hypertension) and DOCA-salt rats (a low renin model of hypertension) suggests that circulating angiotensin II plays a role in local renal NPY gene expression and that the elevated blood pressure per se is not the primary factor responsible for the control of NPY gene expression in the kidney.
Keywords
Animals, Body Weight, Gene Expression, Gene Expression Regulation, Hemodynamics, Hypertension, Renal, Immunohistochemistry, Kidney, Kidney Diseases, Neuropeptide Y, RNA, Messenger, Rats, Rats, Wistar, Renin
Pubmed
Web of science
Create date
05/03/2008 17:40
Last modification date
20/08/2019 16:17
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