The roles of granulocyte-macrophage colony-stimulating factor and interleukin 3 in stromal cell-mediated hemopoiesis in vivo.

Details

Serval ID
serval:BIB_AC9248697744
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The roles of granulocyte-macrophage colony-stimulating factor and interleukin 3 in stromal cell-mediated hemopoiesis in vivo.
Journal
Experimental Hematology
Author(s)
Eliason J.F., Thorens B., Kindler V., Vassalli P.
ISSN
0301-472X[print], 0301-472X[linking]
Publication state
Published
Issued date
05/1988
Volume
16
Number
4
Pages
307-312
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Adherent cells from murine long-term marrow cultures (LTMC) were examined for presence of mRNA for granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 3 (Il-3). Six hours after medium replacement, GM-CSF mRNA was detected but was no longer detectable 24 h after feeding; Il-3 mRNA was not detected at any time. Neutralizing antibodies against these factors had no effect on hemopoiesis. Exogenous Il-3 increased cell production, notably mature erythroid progenitors, whereas GM-CSF had little long-term effect even at high concentrations. Furthermore, GM-CSF appeared to be specifically removed from the medium, whereas virtually all of the Il-3 could be recovered under identical incubation conditions. These results show that Il-3 is not required for maintaining long-term hemopoiesis in vitro, whereas the precise role of GM-CSF in this system remains unclear.
Keywords
Animals, Antibodies/physiology, Bone Marrow/physiology, Bone Marrow Cells, Cell Adhesion, Cells, Cultured, Colony-Forming Units Assay, Colony-Stimulating Factors/immunology, Colony-Stimulating Factors/pharmacology, Granulocyte-Macrophage Colony-Stimulating Factor, Growth Substances/immunology, Growth Substances/pharmacology, Hematopoiesis/drug effects, Interleukin-3/pharmacology, Interleukin-3/physiology, Mice, Mice, Inbred BALB C, RNA, Messenger/analysis
Pubmed
Web of science
Create date
24/01/2008 13:41
Last modification date
20/08/2019 15:16
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