Small, stable shuttle vectors based on the minimal pVS1 replicon for use in gram-negative, plant-associated bacteria.
Details
Serval ID
serval:BIB_AA3A8096AB1C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Small, stable shuttle vectors based on the minimal pVS1 replicon for use in gram-negative, plant-associated bacteria.
Journal
Molecular Plant-microbe Interactions
ISSN
0894-0282 (Print)
ISSN-L
0894-0282
Publication state
Published
Issued date
2000
Volume
13
Number
2
Pages
232-237
Language
english
Abstract
The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.
Keywords
Amino Acid Sequence, Base Sequence, DNA, Recombinant/genetics, Escherichia coli/genetics, Genetic Vectors, Molecular Sequence Data, Plants/microbiology, Plasmids/genetics, Pseudomonas fluorescens/genetics, Replicon/genetics, Sequence Homology, Amino Acid
Pubmed
Web of science
Create date
24/01/2008 14:51
Last modification date
20/08/2019 16:14
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