Article: article from journal or magazin.
Small, stable shuttle vectors based on the minimal pVS1 replicon for use in gram-negative, plant-associated bacteria.
Molecular Plant-microbe Interactions
The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.
Amino Acid Sequence, Base Sequence, DNA, Recombinant/genetics, Escherichia coli/genetics, Genetic Vectors, Molecular Sequence Data, Plants/microbiology, Plasmids/genetics, Pseudomonas fluorescens/genetics, Replicon/genetics, Sequence Homology, Amino Acid
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