High-fat diet consumption alters energy metabolism in the mouse hypothalamus.

Details

Serval ID
serval:BIB_A7BB61E751A8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
High-fat diet consumption alters energy metabolism in the mouse hypothalamus.
Journal
International journal of obesity
Author(s)
Lizarbe B., Cherix A., Duarte JMN, Cardinaux J.R., Gruetter R.
ISSN
1476-5497 (Electronic)
ISSN-L
0307-0565
Publication state
Published
Issued date
06/2019
Peer-reviewed
Oui
Volume
43
Number
6
Pages
1295-1304
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
High-fat diet consumption is known to trigger an inflammatory response in the hypothalamus, which has been characterized by an initial expression of pro-inflammatory genes followed by hypothalamic astrocytosis, microgliosis, and the appearance of neuronal injury markers. The specific effects of high-fat diet on hypothalamic energy metabolism and neurotransmission are however not yet known and have not been investigated before.
We used <sup>1</sup> H and <sup>13</sup> C magnetic resonance spectroscopy (MRS) and immunofluorescence techniques to evaluate in vivo the consequences of high-saturated fat diet administration to mice, and explored the effects on hypothalamic metabolism in three mouse cohorts at different time points for up to 4 months.
We found that high-fat diet increases significantly the hypothalamic levels of glucose (P < 0.001), osmolytes (P < 0.001), and neurotransmitters (P < 0.05) from 2 months of diet, and alters the rates of metabolic (P < 0.05) and neurotransmission fluxes (P < 0.001), and the contribution of non-glycolytic substrates to hypothalamic metabolism (P < 0.05) after 10 weeks of high-fat feeding.
We report changes that reveal a high-fat diet-induced alteration of hypothalamic metabolism and neurotransmission that is quantifiable by <sup>1</sup> H and <sup>13</sup> C MRS in vivo, and present the first evidence of the extension of the inflammation pathology to a localized metabolic imbalance.
Keywords
Animals, Diet, High-Fat/adverse effects, Dietary Fats/administration & dosage, Dietary Fats/pharmacology, Disease Models, Animal, Energy Metabolism/drug effects, Gene Expression Profiling, Hypothalamus/drug effects, Hypothalamus/metabolism, Hypothalamus/physiopathology, Inflammation/metabolism, Mice, Mice, Inbred C57BL, Neurons/metabolism
Pubmed
Web of science
Create date
23/10/2018 9:55
Last modification date
27/04/2020 5:20
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