Localization of the glucosinolate biosynthetic enzymes reveals distinct spatial patterns for the biosynthesis of indole and aliphatic glucosinolates.

Details

Serval ID
serval:BIB_A7B032CB14E1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Localization of the glucosinolate biosynthetic enzymes reveals distinct spatial patterns for the biosynthesis of indole and aliphatic glucosinolates.
Journal
Physiologia Plantarum
Author(s)
Nintemann S.J., Hunziker P., Andersen T.G., Schulz A., Burow M., Halkier B.A.
ISSN
1399-3054 (Electronic)
ISSN-L
0031-9317
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
163
Number
2
Pages
138-154
Language
english
Abstract
Glucosinolates constitute the primary defense metabolites in Arabidopsis thaliana (Arabidopsis). Indole and aliphatic glucosinolates, biosynthesized from tryptophan and methionine, respectively, are known to serve distinct biological functions. Although all genes in the biosynthetic pathways are identified, and it is known where glucosinolates are stored, it has remained elusive where glucosinolates are produced at the cellular and tissue level. To understand how the spatial organization of the different glucosinolate biosynthetic pathways contributes to their distinct biological functions, we investigated the localization of enzymes of the pathways under constitutive conditions and, for indole glucosinolates, also under induced conditions, by analyzing the spatial distribution of several fluorophore-tagged enzymes at the whole plant and the cellular level. We show that key steps in the biosynthesis of the different types of glucosinolates are localized in distinct cells in separate as well as overlapping vascular tissues. The presence of glucosinolate biosynthetic enzymes in parenchyma cells of the vasculature may assign new defense-related functions to these cell types. The knowledge gained in this study is an important prerequisite for understanding the orchestration of chemical defenses from site of synthesis to site of storage and potential (re)mobilization upon attack.
Pubmed
Web of science
Create date
28/06/2018 9:33
Last modification date
20/08/2019 15:12
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