Localization of the glucosinolate biosynthetic enzymes reveals distinct spatial patterns for the biosynthesis of indole and aliphatic glucosinolates.

Détails

ID Serval
serval:BIB_A7B032CB14E1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Localization of the glucosinolate biosynthetic enzymes reveals distinct spatial patterns for the biosynthesis of indole and aliphatic glucosinolates.
Périodique
Physiologia Plantarum
Auteur⸱e⸱s
Nintemann S.J., Hunziker P., Andersen T.G., Schulz A., Burow M., Halkier B.A.
ISSN
1399-3054 (Electronic)
ISSN-L
0031-9317
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
163
Numéro
2
Pages
138-154
Langue
anglais
Résumé
Glucosinolates constitute the primary defense metabolites in Arabidopsis thaliana (Arabidopsis). Indole and aliphatic glucosinolates, biosynthesized from tryptophan and methionine, respectively, are known to serve distinct biological functions. Although all genes in the biosynthetic pathways are identified, and it is known where glucosinolates are stored, it has remained elusive where glucosinolates are produced at the cellular and tissue level. To understand how the spatial organization of the different glucosinolate biosynthetic pathways contributes to their distinct biological functions, we investigated the localization of enzymes of the pathways under constitutive conditions and, for indole glucosinolates, also under induced conditions, by analyzing the spatial distribution of several fluorophore-tagged enzymes at the whole plant and the cellular level. We show that key steps in the biosynthesis of the different types of glucosinolates are localized in distinct cells in separate as well as overlapping vascular tissues. The presence of glucosinolate biosynthetic enzymes in parenchyma cells of the vasculature may assign new defense-related functions to these cell types. The knowledge gained in this study is an important prerequisite for understanding the orchestration of chemical defenses from site of synthesis to site of storage and potential (re)mobilization upon attack.
Pubmed
Web of science
Création de la notice
28/06/2018 10:33
Dernière modification de la notice
20/08/2019 16:12
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