Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43.

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Serval ID
serval:BIB_A71D20B17C23
Type
Article: article from journal or magazin.
Collection
Publications
Title
Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43.
Journal
Cell Communication and Adhesion
Author(s)
Halidi N., Alonso F., Burt J.M., Bény J.L., Haefliger J.A., Meister J.J.
ISSN
1541-9061
ISSN-L
1543-5180
Publication state
Published
Issued date
2012
Volume
19
Number
2
Pages
25-37
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Intercellular Ca(2+) wave propagation between vascular smooth muscle cells (SMCs) is associated with the propagation of contraction along the vessel. Here, we characterize the involvement of gap junctions (GJs) in Ca(2+) wave propagation between SMCs at the cellular level. Gap junctional communication was assessed by the propagation of intercellular Ca(2+) waves and the transfer of Lucifer Yellow in A7r5 cells, primary rat mesenteric SMCs (pSMCs), and 6B5N cells, a clone of A7r5 cells expressing higher connexin43 (Cx43) to Cx40 ratio. Mechanical stimulation induced an intracellular Ca(2+) wave in pSMC and 6B5N cells that propagated to neighboring cells, whereas Ca(2+) waves in A7r5 cells failed to progress to neighboring cells. We demonstrate that Cx43 forms the functional GJs that are involved in mediating intercellular Ca(2+) waves and that co-expression of Cx40 with Cx43, depending on their expression ratio, may interfere with Cx43 GJ formation, thus altering junctional communication.
Keywords
Animals, Calcium Signaling/drug effects, Carbenoxolone/pharmacology, Cell Communication, Cells, Cultured, Connexin 43/genetics, Connexin 43/metabolism, Connexins/genetics, Connexins/metabolism, Fluorescent Dyes/metabolism, Gap Junctions/drug effects, Gap Junctions/metabolism, Gene Expression, Isoquinolines/metabolism, Male, Mesenteric Arteries/cytology, Myocytes, Smooth Muscle/metabolism, Octanols/pharmacology, Peptides/pharmacology, Primary Cell Culture, Protein Binding, Protein Transport, Rats, Rats, Wistar, Single-Cell Analysis
Pubmed
Web of science
Open Access
Yes
Create date
21/01/2013 13:33
Last modification date
20/08/2019 16:11
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