Detection of Gasdermin Activation and Lytic Cell Death During Pyroptosis and Apoptosis

Details

Serval ID
serval:BIB_A6C3ABD5FE08
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
Detection of Gasdermin Activation and Lytic Cell Death During Pyroptosis and Apoptosis
Title of the book
Methods in Molecular Biology
Author(s)
Demarco Benjamin, Ramos Saray, Broz Petr
Publisher
Springer US
ISBN
9781071624487
9781071624494
Publication state
Published
Issued date
2022
Peer-reviewed
Oui
Volume
2523
Pages
209-237
Language
english
Abstract
Cytosolic pattern recognition receptors trigger pyroptosis by detection of danger- or pathogen-associated molecular patterns. These receptors initiate the assembly of inflammasomes, multimeric protein complexes that drive caspase-1 activation. Active caspase-1 cleaves the proinflammatory cytokines IL-1β and IL-18 and the pore-forming protein gasdermin-D (GSDMD) thereby liberating its N-terminal domain. The GSDMD N-termini form multimeric pores at the plasma membrane that allow leakage of intracellular content and ultimately trigger a type of cell death called "pyroptosis." Emerging studies have revealed that GSDMD is also processed by apoptotic caspases-8/-3/-7. In this chapter, we aim to describe methods to monitor lytic cell death and to distinguish between GSDMD processing events and the GSDMD fragments that are generated after pyroptosis or apoptosis induction. We also illustrate the difference between GSDMD pore formation, and final cell lysis, and how this affects to the release of intracellular content. Finally, we show that the activation of another pore-forming protein, gasdermin-E, does not exclusively translate into lytic cell death in bone marrow-derived macrophages.
Keywords
Apoptosis, Caspase 1/metabolism, Inflammasomes/metabolism, Intracellular Signaling Peptides and Proteins/metabolism, Neoplasm Proteins/metabolism, Phosphate-Binding Proteins/metabolism, Pyroptosis, Cell death, Gasdermin, IL-1β quantification, Immunoblotting, Inflammasomes, LDH detection
Pubmed
Web of science
Create date
04/07/2022 11:36
Last modification date
21/10/2023 6:07
Usage data