A versatile and rapid assay of radiosensitivity of peripheral blood leukocytes based on DNA and surface-marker assessment of cytotoxicity
Details
Serval ID
serval:BIB_A60B42B8B18F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A versatile and rapid assay of radiosensitivity of peripheral blood leukocytes based on DNA and surface-marker assessment of cytotoxicity
Journal
Radiation Research
ISSN
0033-7587
Publication state
Published
Issued date
01/1997
Peer-reviewed
Oui
Volume
147
Number
1
Pages
55-60
Notes
Comparative Study
In Vitro
Journal Article --- Old month value: Jan
In Vitro
Journal Article --- Old month value: Jan
Abstract
We describe a rapid assay to predict intrinsic radiosensitivity of normal tissues based on the radiation-induced cytotoxic response of a number of types of white blood cells. Twenty-four hours after irradiation, leukocytes were prepared for analysis by flow cytometry. Radiation-induced cytotoxicity was characterized by degradation of internucleosomal DNA, which results in a reduced G1/G0-phase DNA peak. Clear differences in the radiosensitivity of five of six different types of leukocytes were observed. Significant increases in cytotoxicity were observed even at 0.5 Gy, indicating the technique may be useful as a biological dosimeter after radiation accidents. Based on data from 12 healthy blood donors, significant inter-donor differences in radiosensitivity were observed after exposure to 2 or 8 Gy of high-dose-rate X rays. Inter-donor variation was significantly greater than intra-donor variation for both CD4 and CD8 T lymphocytes (P < 0.01), and the technique yielded adequate data to stratify donors based on the radiosensitivities of these two cell types alone, requiring less than 1 ml of blood. The assay is a general assay of early cytotoxic response to radiation-induced damage and should be useful when assaying the response to chemotherapy as well.
Keywords
Adult
Aged
Antigens, CD/blood
B-Lymphocytes/radiation effects
CD4-Positive T-Lymphocytes/radiation effects
CD8-Positive T-Lymphocytes/radiation effects
Cell Cycle/radiation effects
Cell Survival/radiation effects
DNA/blood/*radiation effects
Dose-Response Relationship, Radiation
Female
Flow Cytometry
G0 Phase
G1 Phase
Granulocytes/radiation effects
Humans
Killer Cells, Natural/radiation effects
Leukocytes/immunology/pathology/*radiation effects
Male
Middle Aged
Monocytes/radiation effects
X-Rays
Pubmed
Web of science
Create date
24/01/2008 17:15
Last modification date
20/08/2019 15:11