MRI monitoring of focal cerebral ischemia in peroxisome proliferator-activated receptor (PPAR)-deficient mice.

Details

Serval ID
serval:BIB_A49581FA0C82
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
MRI monitoring of focal cerebral ischemia in peroxisome proliferator-activated receptor (PPAR)-deficient mice.
Journal
NMR in Biomedicine
Author(s)
Pialat J.B., Cho T.H., Beuf O., Joye E., Moucharrafie S., Moucharaffie S., Langlois J.B., Nemoz C., Janier M., Berthezene Y., Nighoghossian N., Desvergne B., Wiart M.
ISSN
0952-3480[print], 0952-3480[linking]
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
20
Number
3
Pages
335-342
Language
english
Abstract
Peroxisome proliferator-activated receptors (PPARs) are a potential target for neuroprotection in focal ischemic stroke. These nuclear receptors have major effects in lipid metabolism, but they are also involved in inflammatory processes. Three PPAR isotypes have been identified: alpha, beta (or delta) and gamma. The development of PPAR transgenic mice offers a promising tool for prospective therapeutic studies. This study used MRI to assess the role of PPARalpha and PPARbeta in the development of stroke. Permanent middle cerebral artery occlusion induced focal ischemia in wild-type, PPARalpha-null mice and PPARbeta-null mice. T(2)-weighted MRI was performed with a 7 T MRI scan on day 0, 1, 3, 7 and 14 to monitor lesion growth in the various genotypes. General Linear Model statistical analysis found a significant difference in lesion volume between wild-type and PPAR-null mice for both alpha and beta isotypes. These data validate high-resolution MRI for monitoring cerebral ischemic lesions, and confirm the neuroprotective role of PPARalpha and PPARbeta in the brain.
Keywords
Animals, Brain Edema/pathology, Brain Ischemia/chemically induced, Brain Ischemia/diagnosis, Cerebral Infarction/pathology, Diffusion, Magnetic Resonance Imaging, Male, Mice, Mice, Knockout, PPAR alpha/deficiency, PPAR-beta/deficiency, Time Factors
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:26
Last modification date
20/08/2019 15:10
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