MOM22 is a receptor for mitochondrial targeting sequences and cooperates with MOM19.

Details

Serval ID
serval:BIB_A27F1B0DA2B9
Type
Article: article from journal or magazin.
Collection
Publications
Title
MOM22 is a receptor for mitochondrial targeting sequences and cooperates with MOM19.
Journal
EMBO Journal
Author(s)
Mayer A., Nargang F.E., Neupert W., Lill R.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Publication state
Published
Issued date
1995
Peer-reviewed
Oui
Volume
14
Number
17
Pages
4204-4211
Language
english
Abstract
Recognition of targeting signals is a crucial step in protein sorting within the cell. So far, only a few components capable of deciphering targeting signals have been identified, and insights into the chemical nature of the interaction between the signals and their receptors are scarce. Using highly purified mitochondrial outer membrane vesicles, we demonstrate that MOM22 and MOM19, components of the protein import complex of the outer membrane, bind preproteins at the mitochondrial surface in a reversible fashion. Interaction specifically and directly occurs with the N-terminal presequence and is abolished after inactivation of either MOM22 or MOM19. Binding is salt sensitive, suggesting that recognition involves electrostatic forces between the positive charges of the presequence and the acidic cytosolic domain of MOM22. MOM19 and MOM22 can be cross-linked with high efficiency. We propose that the two proteins form a complex which functions as the presequence receptor at the mitochondrial surface and facilitates the movement of preproteins into the translocation pore.
Keywords
Animals, Fungal Proteins, Histidine, Intracellular Membranes/metabolism, Membrane Proteins/biosynthesis, Membrane Proteins/isolation & purification, Membrane Transport Proteins, Mitochondria/metabolism, Models, Structural, Neurospora crassa/metabolism, Protein Precursors/biosynthesis, Protein Precursors/isolation & purification, Receptors, Cell Surface, Receptors, Cytoplasmic and Nuclear/biosynthesis, Receptors, Cytoplasmic and Nuclear/isolation & purification, Recombinant Fusion Proteins/biosynthesis, Recombinant Fusion Proteins/isolation & purification, Reticulocytes/metabolism, Sequence Tagged Sites, Tetrahydrofolate Dehydrogenase/biosynthesis, Tetrahydrofolate Dehydrogenase/isolation & purification
Pubmed
Web of science
Create date
24/01/2008 15:06
Last modification date
20/08/2019 15:08
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