MOM22 is a receptor for mitochondrial targeting sequences and cooperates with MOM19.

Détails

ID Serval
serval:BIB_A27F1B0DA2B9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
MOM22 is a receptor for mitochondrial targeting sequences and cooperates with MOM19.
Périodique
EMBO Journal
Auteur⸱e⸱s
Mayer A., Nargang F.E., Neupert W., Lill R.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
1995
Peer-reviewed
Oui
Volume
14
Numéro
17
Pages
4204-4211
Langue
anglais
Résumé
Recognition of targeting signals is a crucial step in protein sorting within the cell. So far, only a few components capable of deciphering targeting signals have been identified, and insights into the chemical nature of the interaction between the signals and their receptors are scarce. Using highly purified mitochondrial outer membrane vesicles, we demonstrate that MOM22 and MOM19, components of the protein import complex of the outer membrane, bind preproteins at the mitochondrial surface in a reversible fashion. Interaction specifically and directly occurs with the N-terminal presequence and is abolished after inactivation of either MOM22 or MOM19. Binding is salt sensitive, suggesting that recognition involves electrostatic forces between the positive charges of the presequence and the acidic cytosolic domain of MOM22. MOM19 and MOM22 can be cross-linked with high efficiency. We propose that the two proteins form a complex which functions as the presequence receptor at the mitochondrial surface and facilitates the movement of preproteins into the translocation pore.
Mots-clé
Animals, Fungal Proteins, Histidine, Intracellular Membranes/metabolism, Membrane Proteins/biosynthesis, Membrane Proteins/isolation & purification, Membrane Transport Proteins, Mitochondria/metabolism, Models, Structural, Neurospora crassa/metabolism, Protein Precursors/biosynthesis, Protein Precursors/isolation & purification, Receptors, Cell Surface, Receptors, Cytoplasmic and Nuclear/biosynthesis, Receptors, Cytoplasmic and Nuclear/isolation & purification, Recombinant Fusion Proteins/biosynthesis, Recombinant Fusion Proteins/isolation & purification, Reticulocytes/metabolism, Sequence Tagged Sites, Tetrahydrofolate Dehydrogenase/biosynthesis, Tetrahydrofolate Dehydrogenase/isolation & purification
Pubmed
Web of science
Création de la notice
24/01/2008 15:06
Dernière modification de la notice
20/08/2019 15:08
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