A role for LEDGF/p75 in targeting HIV DNA integration

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_A0EA5FE4BC03
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Production externe
Title
A role for LEDGF/p75 in targeting HIV DNA integration
Journal
Nature Medicine
Author(s)
Ciuffi A., Llano M., Poeschla E., Hoffmann C., Leipzig J., Shinn P., Ecker J. R., Bushman F.
ISSN
1078-8956
Publication state
Published
Issued date
12/2005
Peer-reviewed
Oui
Volume
11
Number
12
Pages
1287-9
Language
english
Notes
Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
HIV DNA integration is favored in active genes, but the underlying mechanism is unclear. Cellular lens epithelium-derived growth factor (LEDGF/p75) binds both chromosomal DNA and HIV integrase, and might therefore direct integration by a tethering interaction. We analyzed HIV integration in cells depleted for LEDGF/p75, and found that integration was (i) less frequent in transcription units, (ii) less frequent in genes regulated by LEDGF/p75 and (iii) more frequent in GC-rich DNA. LEDGF is thus the first example of a cellular protein controlling the location of HIV integration in human cells.
Keywords
Adaptor Proteins, Signal Transducing/genetics/*metabolism Blotting, Western Cell Line DNA/*metabolism Gene Silencing HIV/*genetics/physiology HIV Integrase/metabolism Humans Microarray Analysis Transcription Factors/genetics/*metabolism Virus Integration/*physiology
DOI
10.1038/nm1329
Pubmed
16311605
Web of science
000233774400024
Create date
22/02/2008 15:45
Last modification date
17/05/2023 11:31
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