A simple and rapid procedure for the purification of synthetic polypeptides by a combination of affinity chromatography and methionine chemistry.

Details

Serval ID
serval:BIB_9F431BC0DC4C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A simple and rapid procedure for the purification of synthetic polypeptides by a combination of affinity chromatography and methionine chemistry.
Journal
Febs Letters
Author(s)
Roggero M.A., Servis C., Corradin G.
ISSN
0014-5793[print], 0014-5793[linking]
Publication state
Published
Issued date
1997
Volume
408
Number
3
Pages
285-288
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Chemical synthesis of bioactive peptides has become a widespread and rapidly growing technique due to automated and efficient protocols for chain assembly. For most applications, the crude synthetic product must be purified to remove residual reactants, failure sequences and chemically modified peptide species. We propose here a method of universal applicability based on immobilized metal ion affinity chromatography, CNBr cleavage and use of reversible Met-sulfoxide protection. With this method we were able to purify to homogeneity in high yield the PbCS 242-310 polypeptide corresponding to the C-terminal region of Plasmodium berghei CS protein.
Keywords
Amino Acid Sequence, Animals, Chromatography, Affinity/methods, Indicators and Reagents, Methionine/chemistry, Peptides/chemical synthesis, Peptides/chemistry, Plasmodium berghei, Protozoan Proteins/chemical synthesis, Protozoan Proteins/chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:55
Last modification date
20/08/2019 16:05
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