The effect of beef spleen cathepsin-D on pig heart lactate-dehydrogenase
Details
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State: Public
Version: author
Serval ID
serval:BIB_9E4E538C31CA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The effect of beef spleen cathepsin-D on pig heart lactate-dehydrogenase
Journal
Archives of Biochemistry and Biophysics
ISSN
0003-9861
Publication state
Published
Issued date
1975
Peer-reviewed
Oui
Volume
168
Number
1
Pages
252-258
Language
english
Abstract
Pig heart lactate dehydrogenase (EC 1.1.1.27; H4 isoenzyme) has been subjected to the action of beef spleen cathepsin D (EC 3.4.23.5) at pH 3.9. No proteolytic effect on the tetrameric form of the dehydrogenase could be detected. On the other hand, the acid proteinase appears to act on the dissociated monomers in such a manner that they are unable to reassociate into functional tetramers. This finding could be correlated with proteolytic modification of the monomers. The extent of proteolysis depends on the temperature and duration of the incubation.
Keywords
Animals, *Cathepsins, Cattle, Chromatography, Affinity, Chromatography, Gel, Isoenzymes, Kinetics, L-Lactate Dehydrogenase/*metabolism, Macromolecular Substances, Myocardium/*enzymology, NAD/analogs & derivatives, Protein Binding, Protein Denaturation, Spleen/*enzymology, Swine
Pubmed
Web of science
Create date
13/08/2015 8:53
Last modification date
20/08/2019 16:04
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