Inactivation of the Anti-Müllerian Hormone Receptor Type 2 (amhrII) Gene in Northern Pike (Esox lucius) Results in Male-To-Female Sex Reversal.

Details

Serval ID
serval:BIB_9D46CFCAC01A
Type
Article: article from journal or magazin.
Publication sub-type
Letter (letter): Communication to the publisher.
Collection
Publications
Institution
Title
Inactivation of the Anti-Müllerian Hormone Receptor Type 2 (amhrII) Gene in Northern Pike (Esox lucius) Results in Male-To-Female Sex Reversal.
Journal
Sexual development
Author(s)
Pan Q., Herpin A., Guiguen Y.
ISSN
1661-5433 (Electronic)
ISSN-L
1661-5425
Publication state
In Press
Peer-reviewed
Oui
Pages
1-6
Language
english
Notes
Publication types: News
Publication Status: aheadofprint
Abstract
The anti-müllerian hormone (Amh) pathway is crucial for sexual development in teleosts. A male-specific duplicate of anti-müllerian hormone (amhby) was previously identified as the northern pike (Esox lucius) master sex determination gene. However, the role of its putative cognate receptor, i.e., the anti-müllerian hormone receptor type 2 (amhrII) was unclear in this species.
Here, we investigated the role of amhrII during sexual development of northern pike.
We generated stable mutants with deletions in exon 9 of amhrII, inactivating the AmhrII protein using a CRISPR-Cas9-mediated gene knockout strategy.
The inactivation of amhrII in northern pike results in a high level of male-to-female sex reversal.
This result demonstrates that amhrII is necessary for male sexual development in northern pike and supports the idea that AmhrII is a conserved regulator of the teleosts sex differentiation network.
Keywords
Developmental Biology, Embryology, Endocrinology, Diabetes and Metabolism, AMHRII, CRISPR-Cas9, Fish Gene inactivation, Sex determination
Pubmed
Create date
22/03/2022 0:29
Last modification date
08/04/2022 6:36
Usage data