Sodium-dependent phosphate transporters in osteoclast differentiation and function.

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Version: Final published version
Serval ID
serval:BIB_9C533E1C9579
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sodium-dependent phosphate transporters in osteoclast differentiation and function.
Journal
Plos One
Author(s)
Albano G., Moor M., Dolder S., Siegrist M., Wagner C.A., Biber J., Hernando N., Hofstetter W., Bonny O., Fuster D.G.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
04/2015
Peer-reviewed
Oui
Volume
10
Number
4
Pages
e0125104
Language
english
Abstract
Osteoclasts are multinucleated bone degrading cells. Phosphate is an important constituent of mineralized bone and released in significant quantities during bone resorption. Molecular contributors to phosphate transport during the resorptive activity of osteoclasts have been controversially discussed. This study aimed at deciphering the role of sodium-dependent phosphate transporters during osteoclast differentiation and bone resorption. Our studies reveal RANKL-induced differential expression of sodium-dependent phosphate transport protein IIa (NaPi-IIa) transcript and protein during osteoclast development, but no expression of the closely related NaPi-IIb and NaPi-IIc SLC34 family isoforms. In vitro studies employing NaPi-IIa-deficient osteoclast precursors and mature osteoclasts reveal that NaPi-IIa is dispensable for bone resorption and osteoclast differentiation. These results are supported by the analysis of structural bone parameters by high-resolution microcomputed tomography that yielded no differences between adult NaPi-IIa WT and KO mice. By contrast, both type III sodium-dependent phosphate transporters Pit-1 and Pit-2 were abundantly expressed throughout osteoclast differentiation, indicating that they are the relevant sodium-dependent phosphate transporters in osteoclasts and osteoclast precursors. We conclude that phosphate transporters of the SLC34 family have no role in osteoclast differentiation and function and propose that Pit-dependent phosphate transport could be pivotal for bone resorption and should be addressed in further studies.
Pubmed
Web of science
Open Access
Yes
Create date
14/01/2016 9:38
Last modification date
17/09/2020 8:21
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