Transcriptional interference by RNA polymerase III affects expression of the Polr3e gene.

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Ressource 1Download: Genes Dev.-2017-Yeganeh-413-21.pdf (1057.75 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_9C06585B158C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Transcriptional interference by RNA polymerase III affects expression of the Polr3e gene.
Journal
Genes and Development
Author(s)
Yeganeh M., Praz V., Cousin P., Hernandez N.
ISSN
1549-5477 (Electronic)
ISSN-L
0890-9369
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
31
Number
4
Pages
413-421
Language
english
Abstract
Overlapping gene arrangements can potentially contribute to gene expression regulation. A mammalian interspersed repeat (MIR) nested in antisense orientation within the first intron of the Polr3e gene, encoding an RNA polymerase III (Pol III) subunit, is conserved in mammals and highly occupied by Pol III. Using a fluorescence assay, CRISPR/Cas9-mediated deletion of the MIR in mouse embryonic stem cells, and chromatin immunoprecipitation assays, we show that the MIR affects Polr3e expression through transcriptional interference. Our study reveals a mechanism by which a Pol II gene can be regulated at the transcription elongation level by transcription of an embedded antisense Pol III gene.

Keywords
RNA polymerase, gene expression regulation, Polr3e gene, antisense transcription, transcriptional interference, SINE
Pubmed
Web of science
Open Access
Yes
Create date
21/03/2017 19:06
Last modification date
20/08/2019 16:02
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