Dynamic computed tomography with low- and high-molecular-mass contrast agents to assess microvascular permeability modifications in a model of liver fibrosis.

Details

Serval ID
serval:BIB_99D4E782DAA9
Type
Article: article from journal or magazin.
Collection
Publications
Title
Dynamic computed tomography with low- and high-molecular-mass contrast agents to assess microvascular permeability modifications in a model of liver fibrosis.
Journal
Clinical Science (london, England : 1979)
Author(s)
Materne R., Annet L., Dechambre S., Sempoux C., Smith A.M., Corot C., Horsmans Y., Van Beers B.E.
ISSN
0143-5221 (Print)
ISSN-L
0143-5221
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
103
Number
2
Pages
213-216
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Interstitial collagen formation and transformation of the fenestrated hepatic sinusoids into continuous capillaries are major ultrastructural changes that occur in liver cirrhosis and fibrosis. These modifications lead to progressive restriction of blood-liver exchanges. The purpose of our study was to evaluate the permeability changes in a model of hepatic fibrosis by using dynamic computed tomography (CT) enhanced with contrast agents of different molecular masses. Dynamic single-section CT of the liver was performed after intravenous bolus administration of a low-molecular-mass contrast agent (iobitridol) and an experimental high-molecular-mass agent (P840) in normal control rabbits and in rabbits with hepatic fibrosis. Hepatic, aortic and portal venous time-density curves were fitted with a dual-input one-compartmental model to calculate the hepatic mean transit time and distribution volume of the contrast agents. In the rabbits with liver fibrosis, the mean transit time of the high-molecular-mass agent was shorter than that of the low-molecular-mass agent (10.0+/-1.8 s and 12.0+/-1.2 s respectively; P<0.05). The distribution volume accessible to the high-molecular-mass agent was also smaller (22.2+/-4.8% compared with 32.0+/-6.7%; P<0.01). In the normal rabbits, the mean transit times of the high- and low-molecular-mass agents did not differ significantly, and nor did their distribution volumes. Our results demonstrate decreased sinusoidal permeability for the high-molecular-mass agent P840 in a model of hepatic fibrosis. Non-invasive assessment of permeability changes in liver fibrosis can be performed with dynamic CT and contrast agents of different molecular masses.
Keywords
Animals, Capillary Permeability, Contrast Media/pharmacokinetics, Iohexol/analogs & derivatives, Iohexol/pharmacokinetics, Liver/physiopathology, Liver/radiography, Liver Cirrhosis/physiopathology, Liver Cirrhosis/radiography, Male, Molecular Weight, Rabbits, Tomography, X-Ray Computed
Pubmed
Web of science
Create date
20/10/2016 16:29
Last modification date
20/08/2019 15:01
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