Calf rennet lysozyme

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State: Public
Version: author
Serval ID
serval:BIB_98A461BD5DAA
Type
Article: article from journal or magazin.
Collection
Publications
Title
Calf rennet lysozyme
Journal
Biochemical Journal
Author(s)
Pahud J.J., Widmer F.
ISSN
0264-6021
Publication state
Published
Issued date
1982
Peer-reviewed
Oui
Volume
201
Number
3
Pages
661-664
Language
english
Abstract
A glycosidase displaying endo-N-acetylmuramoylhydrolase specificity (EC 3.2.1.17) was isolated from calf rennet. This lysozyme was also present in abomasal secretions from calf and adult cattle. Multiple molecular forms revealed by electrofocusing might be artefacts. The main enzyme form had Mr approx. 15 000, pH optimum 5.0, pI7.5, and a remarkable conformation stability. Competitive inhibition was observed with both N-acetylglucosamine and N-acetylmuramic acid, with apparent Ki values of 29 mM and 2.4 mM respectively. The isolated enzyme also displayed significant chitinase activity.
Keywords
Animals, Cattle, Chymosin/*analysis, Hydrogen-Ion Concentration, Isoenzymes/isolation & purification, Kinetics, Muramidase/isolation & purification/*metabolism, Protein Conformation
Pubmed
Web of science
Create date
13/08/2015 9:01
Last modification date
20/08/2019 16:00
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