A geographic variant of the Staphylococcus aureus Panton-Valentine leukocidin toxin and the origin of community-associated methicillin-resistant S. aureus USA300.

Details

Serval ID
serval:BIB_9751E0EB1455
Type
Article: article from journal or magazin.
Collection
Publications
Title
A geographic variant of the Staphylococcus aureus Panton-Valentine leukocidin toxin and the origin of community-associated methicillin-resistant S. aureus USA300.
Journal
The Journal of infectious diseases
Author(s)
O'Hara F.P., Guex N., Word J.M., Miller L.A., Becker J.A., Walsh S.L., Scangarella N.E., West J.M., Shawar R.M., Amrine-Madsen H.
ISSN
0022-1899 (Print)
ISSN-L
0022-1899
Publication state
Published
Issued date
15/01/2008
Peer-reviewed
Oui
Volume
197
Number
2
Pages
187-194
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The majority of recent community-associated methicillin-resistant Staphylococcus aureus (MRSA) infections in the United States have been caused by a single clone, USA300. USA300 secretes Panton-Valentine leukocidin (PVL) toxin, which is associated with highly virulent infections.
We sequenced the PVL genes of 174 S. aureus isolates from a global clinical sample. We combined phylogenetic reconstruction and protein modeling methods to analyze genetic variation in PVL.
Nucleotide variation was detected at 12 of 1726 sites. Two PVL sequence variants, the R variant and the H variant, were identified on the basis of a substitution at nt 527. Of sequences obtained in the United States, 96.7% harbor the R variant, whereas 95.6% of sequences obtained outside the United States harbor the H variant; 91.3% of MRSA isolates harbor the R variant, and 91.3% of methicillin-susceptible strains harbor the H variant. A molecular model of PVL shows 3 mechanisms by which the amino acid substitution may affect PVL function.
All sampled PVL genes appear to share a recent common ancestor and spread via a combination of clonal expansion and horizontal transfer. US isolates harbor a variant of PVL that is strongly associated with MRSA infections. Protein modeling reveals that this variant may have functional significance. We propose a hypothesis for the origin of USA300.
Keywords
Adult, Amino Acid Substitution, Bacterial Toxins/chemistry, Bacterial Toxins/genetics, Child, Child, Preschool, Community-Acquired Infections/microbiology, Evolution, Molecular, Exotoxins/chemistry, Exotoxins/genetics, Gene Transfer, Horizontal, Genetic Variation, Humans, Leukocidins/chemistry, Leukocidins/genetics, Methicillin/pharmacology, Methicillin Resistance, Models, Molecular, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Staphylococcal Infections/microbiology, Staphylococcus aureus/classification, Staphylococcus aureus/drug effects, Staphylococcus aureus/genetics
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2021 15:26
Last modification date
30/01/2021 6:26
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