A geographic variant of the Staphylococcus aureus Panton-Valentine leukocidin toxin and the origin of community-associated methicillin-resistant S. aureus USA300.

Détails

ID Serval
serval:BIB_9751E0EB1455
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A geographic variant of the Staphylococcus aureus Panton-Valentine leukocidin toxin and the origin of community-associated methicillin-resistant S. aureus USA300.
Périodique
The Journal of infectious diseases
Auteur(s)
O'Hara F.P., Guex N., Word J.M., Miller L.A., Becker J.A., Walsh S.L., Scangarella N.E., West J.M., Shawar R.M., Amrine-Madsen H.
ISSN
0022-1899 (Print)
ISSN-L
0022-1899
Statut éditorial
Publié
Date de publication
15/01/2008
Peer-reviewed
Oui
Volume
197
Numéro
2
Pages
187-194
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The majority of recent community-associated methicillin-resistant Staphylococcus aureus (MRSA) infections in the United States have been caused by a single clone, USA300. USA300 secretes Panton-Valentine leukocidin (PVL) toxin, which is associated with highly virulent infections.
We sequenced the PVL genes of 174 S. aureus isolates from a global clinical sample. We combined phylogenetic reconstruction and protein modeling methods to analyze genetic variation in PVL.
Nucleotide variation was detected at 12 of 1726 sites. Two PVL sequence variants, the R variant and the H variant, were identified on the basis of a substitution at nt 527. Of sequences obtained in the United States, 96.7% harbor the R variant, whereas 95.6% of sequences obtained outside the United States harbor the H variant; 91.3% of MRSA isolates harbor the R variant, and 91.3% of methicillin-susceptible strains harbor the H variant. A molecular model of PVL shows 3 mechanisms by which the amino acid substitution may affect PVL function.
All sampled PVL genes appear to share a recent common ancestor and spread via a combination of clonal expansion and horizontal transfer. US isolates harbor a variant of PVL that is strongly associated with MRSA infections. Protein modeling reveals that this variant may have functional significance. We propose a hypothesis for the origin of USA300.
Mots-clé
Adult, Amino Acid Substitution, Bacterial Toxins/chemistry, Bacterial Toxins/genetics, Child, Child, Preschool, Community-Acquired Infections/microbiology, Evolution, Molecular, Exotoxins/chemistry, Exotoxins/genetics, Gene Transfer, Horizontal, Genetic Variation, Humans, Leukocidins/chemistry, Leukocidins/genetics, Methicillin/pharmacology, Methicillin Resistance, Models, Molecular, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Staphylococcal Infections/microbiology, Staphylococcus aureus/classification, Staphylococcus aureus/drug effects, Staphylococcus aureus/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2021 15:26
Dernière modification de la notice
30/01/2021 6:26
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