Stress reaction of kidney epithelial cells to inorganic solid-core nanoparticles.

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Serval ID
serval:BIB_961C1B369FAB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Stress reaction of kidney epithelial cells to inorganic solid-core nanoparticles.
Journal
Cell Biology and Toxicology
Author(s)
Halamoda Kenzaoui B., Chapuis Bernasconi C., Juillerat-Jeanneret L.
ISSN
1573-6822 (Electronic)
ISSN-L
0742-2091
Publication state
Published
Issued date
01/2013
Volume
29
Number
1
Pages
39-58
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
A route of accumulation and elimination of therapeutic engineered nanoparticles (NPs) may be the kidney. Therefore, the interactions of different solid-core inorganic NPs (titanium-, silica-, and iron oxide-based NPs) were studied in vitro with the MDCK and LLC-PK epithelial cells as representative cells of the renal epithelia. Following cell exposure to the NPs, observations include cytotoxicity for oleic acid-coated iron oxide NPs, the production of reactive oxygen species for titanium dioxide NPs, and cell depletion of thiols for uncoated iron oxide NPs, whereas for silica NPs an apparent rapid and short-lived increase of thiol levels in both cell lines was observed. Following cell exposure to metallic NPs, the expression of the tranferrin receptor/CD71 was decreased in both cells by iron oxide NPs, but only in MDCK cells by titanium dioxide NPs. The tight association, then subsequent release of NPs by MDCK and LLC-PK kidney epithelial cells, showed that following exposure to the NPs, only MDCK cells could release iron oxide NPs, whereas both cells released titanium dioxide NPs. No transfer of any solid-core NPs across the cell layers was observed.
Keywords
Cytotoxicity, Epithelial kidney cells, Nanoparticles, Oxidative stress, Transepithelial transfer, Transferrin receptor/CD71
Pubmed
Web of science
Open Access
Yes
Create date
10/01/2013 15:04
Last modification date
01/10/2019 6:18
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