Stress reaction of kidney epithelial cells to inorganic solid-core nanoparticles.
Détails
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Etat: Public
Version: Final published version
Licence: Non spécifiée
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Etat: Public
Version: Final published version
Licence: Non spécifiée
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
ID Serval
serval:BIB_961C1B369FAB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Stress reaction of kidney epithelial cells to inorganic solid-core nanoparticles.
Périodique
Cell Biology and Toxicology
ISSN
1573-6822 (Electronic)
ISSN-L
0742-2091
Statut éditorial
Publié
Date de publication
01/2013
Volume
29
Numéro
1
Pages
39-58
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
A route of accumulation and elimination of therapeutic engineered nanoparticles (NPs) may be the kidney. Therefore, the interactions of different solid-core inorganic NPs (titanium-, silica-, and iron oxide-based NPs) were studied in vitro with the MDCK and LLC-PK epithelial cells as representative cells of the renal epithelia. Following cell exposure to the NPs, observations include cytotoxicity for oleic acid-coated iron oxide NPs, the production of reactive oxygen species for titanium dioxide NPs, and cell depletion of thiols for uncoated iron oxide NPs, whereas for silica NPs an apparent rapid and short-lived increase of thiol levels in both cell lines was observed. Following cell exposure to metallic NPs, the expression of the tranferrin receptor/CD71 was decreased in both cells by iron oxide NPs, but only in MDCK cells by titanium dioxide NPs. The tight association, then subsequent release of NPs by MDCK and LLC-PK kidney epithelial cells, showed that following exposure to the NPs, only MDCK cells could release iron oxide NPs, whereas both cells released titanium dioxide NPs. No transfer of any solid-core NPs across the cell layers was observed.
Mots-clé
Cytotoxicity, Epithelial kidney cells, Nanoparticles, Oxidative stress, Transepithelial transfer, Transferrin receptor/CD71
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/01/2013 16:04
Dernière modification de la notice
14/02/2022 8:56